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Trehalose Purification

Water Treatment
Membrane Separation
Continuous Ion Exchange
Continuous Chromatography

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Trehalose Purification

Trehalose Purification

 

 

1.      Feed Condition

 

Basic   data

Feed

3.5m³/hour30DS (%W/W) trehalose   fermentation broth

Feed solid content

DS30

Trehalose fraction

60-65%

pH

3.8-4.5

Operating temperature

55-60

Technical   indicators

Amount of resin

~32-35m3

Trehalose product flow

~6m3/hour

Trehalose purity

≥90%

Trehalose liquid-solid content

≥10

Fructose flow

~4.8m3/hour

Fructose DS

≥10

Fructose purity

≥90%

Recycling water

~13m3/hour (Trehalose balance area)

  

2. Purification Process

The mixture of trehalose and glucose passes through the chromatographic separation system filled with chromatographic resin. In the resin tank, since the chromatographic resin has different forces to trehalose and glucose, trehalose flows out faster than glucose does. In addition, due to the acting force of chromatographic resin, glucose flows relatively slow and should be eluted with water. The subsequent effluent is glucose. In order to further improve the separation effect, a part of glucose solution should be mixed with the initially separated feed, and then separated through the resin in order to collect high-purity trehalose solution.

 

 

4. Operating Cost

 

The total annual operating cost of equipment

177

10,000 yuan

glycine output

27720

ton/year

Unit processing cost

63.8

yuan/ton

90%Trehalose output

4125

ton/year

90%glucose output

3300

ton/year

unit consumption of

electricity capacity(0.7kwh)

20.2

yuan/ton

Amount of processing pure water unit consumption

42.3

yuan/ton

Amount of processing resin unit consumption

1.3

yuan/ton

 

 

 

Typical applications